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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Increased Collagen Turnover Impairs Tendon Microstructure and Stability in Integrin α2β1-Deficient Mice
doi: 10.3390/ijms21082835
Figure Lengend Snippet: Resident MMP-2 activity is increased in integrin α2β1-deficient tendons. Gelatin zymography of 30-µg tendon lysate. Quantification of the MMP-2-activity signal ( A ). Zymography gel 10% acrylamide with polymerized gelatin. The arrows depict the pro and active forms of MMP-9 and MMP-2, respectively ( B ). C-terminal fragments (CTX) ELISA quantification of soluble collagen fragments in the tendon ( C ) (N = 5). Mann-Whitney U test was used for statistical testing (** p < 0.01).
Article Snippet: Insoluble, intact collagen fibrils were removed from the lysate by centrifugation for 10 min at 13.200× g . The samples were diluted 1:10, and a
Techniques: Activity Assay, Zymography, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY